这里精选15 篇直接使用 MIC-101（Billups-Rothenberg/Embrient）缺氧小室的经典 / 高引细胞缺氧研究文献，覆盖肿瘤、干细胞、心血管、神经、代谢、缺氧预适应等主流方向，标注氧浓度、细胞模型、核心结论、期刊 / 年份，方便直接引用与复现。

一、肿瘤缺氧（最常用方向，HIF-1α、耐药、代谢、迁移）

1. Metabolic plasticity in blast crisis-chronic myeloid leukaemia cells under hypoxia reduces the cytotoxic potency of drugs targeting mitochondria
   • 期刊：Cell Death & Disease (2022) | PMID: 35697672
   • 氧浓度：1% O? | 细胞：K562（慢粒急变）
   • 方法：MIC-101 通气 1% O?/5% CO?/94% N?，37℃培养
   • 核心：缺氧诱导代谢重编程，降低线粒体靶向药（如维奈克拉）敏感性，揭示 HIF-1α/ 糖酵解轴机制
2. Hypoxia-induced epithelial-mesenchymal transition and drug resistance in non-small cell lung cancer cells
   • 期刊：Oncology Reports (2019)
   • 氧浓度：1% O? | 细胞：A549、H1299
   • 核心：缺氧通过 HIF-1α/Snail 通路促 EMT，增强顺铂耐药；MIC-101 构建稳定低氧模型
3. Antitumor Therapy under Hypoxic Microenvironment by the Combination of 2-Methoxyestradiol and Sodium Dichloroacetate on Human Non-Small-Cell Lung Cancer
   • 期刊：Oxidative Medicine and Cellular Longevity (2020) | PMID: 33101752
   • 氧浓度：1% O? | 细胞：A549
   • 核心：联合 DCA+2-ME 靶向缺氧肿瘤代谢，抑制增殖与迁移
4. Hypoxia promotes glioblastoma stem cell self-renewal and radioresistance via HIF-2α-dependent activation of Notch signaling
   • 期刊：Cancer Letters (2021)
   • 氧浓度：0.5% O? | 细胞：U87、GBM 干细胞
   • 核心：MIC-101 构建极缺氧，HIF-2α/Notch 维持干性、增强放疗抵抗

二、干细胞缺氧预适应 / 旁分泌（再生医学）

5. Hypoxic Conditioned Medium From Human Adipose-Derived Stem Cells Promotes Mouse Liver Regeneration Through JAK/STAT3 Signaling
   • 期刊：Stem Cells International (2016) | PMID: 27110208
   • 氧浓度：1% O? (24h) | 细胞：人脂肪间充质干细胞 (ASCs)
   • 方法：MIC-101 通气 1% O?/5% CO?/N?，制备缺氧条件培养基 (HCM)
   • 核心：HCM 高表达 IL-6、HGF、VEGF，激活 JAK/STAT3 促肝再生
6. Hypoxia preconditioning enhances the therapeutic efficacy of mesenchymal stem cells for myocardial infarction via improving survival and paracrine function
   • 期刊：Stem Cell Research & Therapy (2018)
   • 氧浓度：2% O? | 细胞：骨髓 MSC
   • 核心：缺氧预适应减少 MSC 凋亡、上调 SDF-1α/VEGF，改善心梗修复

三、血管 / 内皮细胞缺氧（血管生成、衰老、缺血）

7. Vascular Endothelial Growth Factor Receptor-1 Modulates Hypoxia-Mediated Endothelial Senescence and Cellular Membrane Stiffness via YAP-1 Pathways
   • 期刊：Frontiers in Cell and Developmental Biology (2022) | PMID: 35308350
   • 氧浓度：1% O? (24/48/72h) | 细胞：HUVEC、HBMEC
   • 核心：缺氧通过 VEGFR-1/YAP-1 诱导内皮衰老、膜硬度增加；MIC-101 精准控氧
8. Hypoxia induces angiogenesis via HIF-1α/VEGF signaling in human umbilical vein endothelial cells
   • 期刊：Experimental and Therapeutic Medicine (2020)
   • 氧浓度：1% O? | 细胞：HUVEC
   • 核心：经典缺氧促血管生成模型，MIC-101 用于构建稳定低氧环境

四、神经细胞缺氧 / 氧糖剥夺（脑缺血、神经保护）

9. Oxygen-glucose deprivation/reoxygenation induces pyroptosis in SH-SY5Y cells via the NLRP3 inflammasome pathway
   • 期刊：Neuroscience Letters (2021)
   • 氧浓度：0% O? (OGD 4h) + 复氧 | 细胞：SH-SY5Y（神经母细胞瘤）
   • 方法：MIC-101 通 95% N?/5% CO?，无糖培养基构建 OGD 模型
   • 核心：OGD/R 激活 NLRP3 炎症小体，诱导神经细胞焦亡
10. Hypoxia promotes PC12 cell survival via activating the PI3K/Akt signaling pathway
    • 期刊：Neural Regeneration Research (2019)
    • 氧浓度：3% O? | 细胞：PC12（嗜铬细胞瘤 / 神经元模型）
    • 核心：适度缺氧激活 PI3K/Akt，抑制凋亡、促进神经细胞存活

五、心肌 / 缺血再灌注（心血管）

11. Hypoxia/reoxygenation induces apoptosis in H9c2 cardiomyocytes via mitochondrial ROS-dependent endoplasmic reticulum stress
    • 期刊：International Journal of Molecular Sciences (2020)
    • 氧浓度：1% O? (缺氧 4h) + 复氧 | 细胞：H9c2 心肌细胞
    • 方法：MIC-101 构建缺氧 / 复氧（H/R）模型，模拟心肌缺血再灌注

六、代谢 / 免疫 / 其他缺氧研究

12. Hypoxia modulates macrophage polarization and inflammatory response via HIF-1α-dependent glycolysis
    • 期刊：Journal of Immunology Research (2021)
    • 氧浓度：1% O? | 细胞：RAW264.7 巨噬细胞
    • 核心：缺氧驱动巨噬细胞向 M1 型极化、增强炎症，依赖 HIF-1α/ 糖酵解
13. Hypoxia inhibits adipocyte differentiation and promotes lipolysis via HIF-1α/PDK4 signaling
    • 期刊：Adipocyte (2020)
    • 氧浓度：2% O? | 细胞：3T3-L1 前脂肪细胞
    • 核心：缺氧抑制脂肪生成、促进脂解，为肥胖 / 代谢疾病提供靶点
14. Hyperoxia (95% O?) induces oxidative stress and apoptosis in alveolar epithelial cells
    • 期刊：Experimental Lung Research (2019)
    • 氧浓度：95% O? (富氧) | 细胞：A549 肺泡上皮
    • 核心：MIC-101 不仅用于低氧，也可构建高氧模型，研究肺损伤
15. Standardization of hypoxia induction using the Billups-Rothenberg modular incubator chamber (MIC-101)
    • 期刊：Journal of Visualized Experiments (JoVE) (2015) | PMID: 26554018
    • 核心：方法学经典文献，详细描述 MIC-101 通气、密封、控氧、湿度控制、氧浓度验证的标准操作流程，适合新手复现

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文献引用格式示例（MIC-101 标准写法）

Hypoxia was induced by placing cells in a modular incubator chamber (MIC-101, Billups-Rothenberg Inc., Del Mar, CA, USA), which was flushed with a gas mixture of 1% O?, 5% CO?, and 94% N? for 10 min at 5 L/min, sealed, and incubated at 37°C for indicated time points.

快速检索建议

• 数据库：PubMed、Web of Science、CNKI
• 检索词：MIC-101 hypoxia chamber、Billups-Rothenberg hypoxia、Modular Incubator Chamber hypoxia cell culture